Epstein-BarrVirusDNAExacerbatesColitisSymptomsinaMouseModelofInflammatoryBowelDisease.
EB病毒DNA加剧IBD小鼠模型结肠炎症状。
Abstract
InfectionwithEBVhasbeenassociatedwithvariousinflammatorydisordersincludinginflammatoryboweldiseases(IBD).Contributionofthisvirustointestinaldiseaseprocesseshasnotbeenassessed.WepreviouslydetectedthatEBVDNAtriggersproinflammatoryresponsesviatheactivationofendosomalToll-likereceptor(TLR)signaling.Hence,toexaminethecolitogenicpotentialofEBVDNA,weusedthedextransodiumsulfate(DSS)mousecolitismodel.C57BL/6JmicereceivedeitherDSS-containingorregulardrinkingwater.MicewerethenadministeredEBVDNAbyrectalgavage.AdministrationofEBVDNAtotheDSS-fedmiceaggravatedcolonicdiseaseactivityaswellasincreasedthedamagetothecolonhistologicarchitecture.
EBV感染与各种炎症性疾病有关，包括IBD。目前尚未评估该病毒在肠道疾病过程中的作用。我们之前检测到EBVDNA通过激活TLR信号触发促炎反应。因此，为了验证EBVDNA在结肠炎方面的作用，我们采用了DSS诱导的小鼠结肠炎模型。予C57BL/6J小鼠含DSS或常规饮用水，然后通过直肠管饲法给小鼠注射EBVDNA。向DSS喂养的小鼠予EBVDNA会加重结肠疾病活动度，并增加对结肠组织结构的损害。
Moreover,weobservedenhancedexpressionofIL-17A,IFNγandTNFαincolontissuesfromthecolitismice(DSS-treated)giventheEBVDNA
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